Journal: Chinese Medicine

Article Title: A novel Chinese medicine formula against hyperuricemia-induced kidney inflammation involving pyroptosis inhibition via modulating AMPK-TLR4-NLRP3 pathway

doi: 10.1186/s13020-025-01271-2

Figure Lengend Snippet: Chemical compositions and quality control method and the therapeutic effects of GF on the severity of HUA in the HUA mice. A Chemical compositions of GF. B Chromatogram of GF and alisol B 23-acetate detected at 203 nm. C Chromatogram of GF and paeoniflorin detected at 230 nm. D Chromatogram of GF and astilbin detected at 288 nm. E Representative images of the kidneys of the HUA mice. F Pathological changes in the kidney tissues using H&E staining (scale bar represents 50 µm). G The kidney index; H SUA; I Urine UA; J BUN and K CRE in serum; L XOD activity in the liver; M URAT1 in the kidneys. N and O Representative western blotting images and quantitative analysis of the protein expressions of GLUT9, URAT1, OAT1, and OAT3 in the kidneys of the HUA mice. All data were expressed as mean ± SEM ( G – M , n = 8–10; N – O , n = 3–4). # p < 0.05 and ## p < 0.01 compared to the control group; * p < 0.05 and ** p < 0.01 compared to the PO + HX vehicle control group

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h and incubated overnight at 4 °C with primary antibodies against AMPK (ABclonal, A12491), p-AMPK (ABclonal, AP0432), TLR4 (CST, #14,358; Servicebio, GB11519-100), MYD88 (Servicebio, GB115549-100), NF-κB p65 (Abcam, ab32536; CST, #8242; Servicebio, GB11997-100), p-p65 (Servicebio, GB113882-100), IκBα (GB151509-100; GB11212-100), p-IκBα (CST, #2859), NLRP3 (GB114320-100), ASC (Affinty, DF6304; Servicebio, GB115270-100; GB113966-100), TNF-α (Servicebio, GB11188-100), Pro-caspase-1(Abcam, ab179515), Caspase-1 (ABclonal, A21085; Servicebio, GB11383-100), GSDMD-NT (Affinty, #DF13758), OAT1 (ABclonal, A1814; Servicebio, GB113690-100), OAT3 (ABclonal, A14575), URAT1 (ABclonal, A5118; Servicebio, GB111847-100), GLUT9 (ABclonal, A14592), and β-actin (Santa Cruz, SC69879), and all of which were diluted to 1:1000 in 5% BSA-TBST.

Techniques: Control, Staining, Activity Assay, Western Blot

Journal: Chinese Medicine

Article Title: A novel Chinese medicine formula against hyperuricemia-induced kidney inflammation involving pyroptosis inhibition via modulating AMPK-TLR4-NLRP3 pathway

doi: 10.1186/s13020-025-01271-2

Figure Lengend Snippet: GF reduces UA level in the MSU-stimulated HK-2 cells and inhibited pyroptosis by regulating the AMPK-TLR4-NLRP3 pathway. A Cell viability. B Images of the HUA HK-2 cell pyroptosis. C , D Flow cytometry analysis for TUNEL staining in HK-2 cells (n ≥ 3 per group, all experiments were performed in triplicate). E Level of LDH release in HK-2 cells. F – G Intracellular and extracellular UA levels. H the in vitro XOD inhibition. I Localization of HMGB1 detected by IF staining (scale bar represents 20 μm, HMGB1 green, DAPI blue). J – K Representative western blotting images and quantitative analysis of the protein expression of GLUT9, URAT1, OAT1, and OAT3 in the HUA HK-2 cells. L – X Representative western blotting images and quantitative analysis of the protein expression of AMPK-TLR4-NLRP3 pathway, including p-AMPK/AMPK, TLR4, MYD88, p-IκBα/IκBα, p-NFκB-p65/NFκB-p65, NLRP3, GSDMD-NT, TNF-α, ASC, Caspase-1 in the MSU-stimulated HK-2 cells. All data were expressed as mean ± SEM ( B – H , n = 8–10; J – X , n = 3–5). # p < 0.05 and ## p < 0.01 compared to the control group; * p < 0.05 and ** p < 0.01 compared to the MSU vehicle control group

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h and incubated overnight at 4 °C with primary antibodies against AMPK (ABclonal, A12491), p-AMPK (ABclonal, AP0432), TLR4 (CST, #14,358; Servicebio, GB11519-100), MYD88 (Servicebio, GB115549-100), NF-κB p65 (Abcam, ab32536; CST, #8242; Servicebio, GB11997-100), p-p65 (Servicebio, GB113882-100), IκBα (GB151509-100; GB11212-100), p-IκBα (CST, #2859), NLRP3 (GB114320-100), ASC (Affinty, DF6304; Servicebio, GB115270-100; GB113966-100), TNF-α (Servicebio, GB11188-100), Pro-caspase-1(Abcam, ab179515), Caspase-1 (ABclonal, A21085; Servicebio, GB11383-100), GSDMD-NT (Affinty, #DF13758), OAT1 (ABclonal, A1814; Servicebio, GB113690-100), OAT3 (ABclonal, A14575), URAT1 (ABclonal, A5118; Servicebio, GB111847-100), GLUT9 (ABclonal, A14592), and β-actin (Santa Cruz, SC69879), and all of which were diluted to 1:1000 in 5% BSA-TBST.

Techniques: Flow Cytometry, TUNEL Assay, Staining, In Vitro, Inhibition, Western Blot, Expressing, Control

Journal: Frontiers in Nutrition

Article Title: Guiling prescription attenuates hyperuricemia via multi-target regulation of uric acid metabolism, renal protection, and inflammation: insights from metabolomics and network pharmacology

doi: 10.3389/fnut.2025.1738623

Figure Lengend Snippet: GP promoted UA excretion through modulation of renal UA transporters in HUA rats. (A) Expression of ABCG2, OAT1, OAT3, and GLUT9, URAT1 in rat kidney. (B–F) The western blot analysis of ABCG2, OAT1, OAT3, and GLUT9, URAT1 relative protein expression levels. Data are presented as mean ± S.E.M ( n = 3). ## P < 0.01 vs. Con; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. Mod.

Article Snippet: Antibodies for ATP binding cassette subfamily G member 2 (ABCG2), organic anion transporter 1 (OAT1), organic anion transporter 3 (OAT3), urate anion transporter 1 (URAT1), and glucose transporter 9 (GLUT9) were provided by ABclonal (Wuhan, China).

Techniques: Expressing, Western Blot